Product Information

Contents: Phycoerythrin (PE) anti-mouse Dendritic Cell Marker (33D1)
Catalog Number: 12-5884
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 33D1
Isotype: Rat IgG2b, κ

Staining of BALB/c splenocytes with FITC anti-mouse MHC Class II (M5/114.15.2) (cat. 11-5321) and 0.125 μg of PE Rat IgG2b Iso Cntrl (cat. 12-4032) (left) or 0.125 μg of PE 33D1 (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5884	Affinity Purified anti-mouse Dendritic Cell (DC) Marker (33D1)	N/A	N/A	FA FC IH/F
13-5884	Biotin anti-mouse Dendritic Cell (DC) Marker (33D1)	N/A	N/A	FC
12-5884	PE anti-mouse Dendritic Cell (DC) Marker (33D1)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 33D1 monoclonal antibody reacts with a mouse DC-specific surface marker. The nature and biological activity of the 33D1 antigen is as of yet unknown. 33D1 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer’s patch. Bone marrow dendritic cells require GM-CSF to express the 33D1 antigen and this expression is down regulated in the presence of IL-4. 33D1 antigen has been detected in vivo in brain dendritic cells post infection via Toxoplasma gondii.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 33D1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nussenzweig, M.C., R.M. Steinman, M.D. Witmer, and B. Gutchinov. 1982. A monoclonal antibody specific for mouse dendritic cells. Proc. Natl. Acad. Sci. USA 79: 161 - 165.

Steinman, R.M., B. Gutchinov, M.D. Witmer, and M.C. Nussenzweig. 1983. Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice. J. Exp. Med. 157: 613 - 627.

Inaba, K., M. Inaba, N. Romani, H. Aya, M. Deguchi, S. Ikehara, S. Muramatsu, and R.M. Steinman. 1992. Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 176: 1693 - 1702.

Masurier, C., C. Pioche-Durieu, B.M. Colombo, R. Lacave, F.M. Lemoine, D. Klatzmann, and M. Guigon. 1999. Immunophenotypical and functional heterogeneity of dendritic cells generated from murine bone marrow cultured with different cytokine combinations: implications for anti-tumoral cell therapy. Immunology 96: 569 - 577.

Fischer, H.-G., U. Bonifas, and G. Reichmann. 2000. Phenotype and functions of brain dendritic cells emerging during chronic infection of mice with Toxoplasma gondii. J. Immunol. 164: 4826 - 4834.

Dudziak D, Kamphorst AO, et al. 2007. Differential antigen processing by dendritic cell subsets in vivo. Science 315(5808):107-11. (33D1, IHC frozen, PubMed)

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