Contents: Phycoerythrin (PE) anti-human AITR/GITR
Catalog Number: 12-5875
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioAITR
Isotype: Mouse IgG1
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Staining of human PBMC, cultured for 3 days with 5 μg/ml PHA, with FITC anti-human ICOS (ISA-3) (cat. 11-9948) and 20μl of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or 20μl of PE anti-human AITR/GITR (eBioAITR) (right). Total viable cells were used for analysis.
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Description
The monoclonal antibody reacts with human AITR, Activation Inducible TNFR family member, an approximately 25 kDa member of the TNFR superfamily. AITR mRNA is detected in lymph node, peripheral blood leukocytes and weakly in spleen. AITR mRNA expression was upregulated within 24 hour on PMA/ionomycin or PHA stimulated PBMC. At the protein level, AITR is expressed by a small population of activated PBMC. AITR associates with TRAF1, TRAF2 and TRAF3 and induces nuclear factor NF-κB activation via TRAF2. Recently TL6 (AITRL) has been reported as the ligand for AITR. Interaction of AITR with AITRL is important for cross-talk between T lymphocytes and endothelial cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioAITR antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioAITR antibody has been pre-titrated and tested by flow cytometric analysis of PHA-stimulated human peripheral blood mononuclear cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.
References
Kwon B, Yu KY, Ni J, Yu GL, Jang IK, Kim YJ, Xing L, Liu D, Wang SX, Kwon BS. 1999. J Biol Chem. 274(10):6056-61. Identification of a novel activation-inducible protein of the tumor necrosis factor receptor superfamily and its ligand.
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Human Cellular Antigens Chart