Product Information

Contents: Phycoerythrin (PE) anti-mouse GITR (TNFRSF18)
Catalog Number: 12-5874
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DTA-1
Isotype: Rat IgG2b

Staining of C57Bl/6 splenocytes with APC anti-mouse CD25 (PC61.5) (cat. 17-0251) and 0.06 μg of PE Rat IgG2b Isotype Control (cat. 12-4031) (left) or 0.002 μg of PE anti-mouse GITR (DTA-1) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5874	Affinity Purified anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FA FC IP
16-5874	Functional Grade* Purified anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FA FC
13-5874	Biotin anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FC
11-5874	Fluorescein isothiocyanate (FITC) anti-mouse GITR (TNFRSF18, AITR)	488	518	FC
12-5874	Phycoerythrin (PE) anti-mouse GITR (TNFRSF18, AITR)	488	575	FC
17-5874	Allophycocyanin (APC) anti-mouse GITR (TNFRSF18, AITR)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naïve mice, GITR is expressed predominantly by CD4⁺CD25⁺ T regulatory cells (Treg) and by CD25⁺ CD4⁺ CD8^- thymocytes. Stimulation of GITR abrogates Treg cell-mediated suppression. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DTA-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.004 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Note: The concentration suggested above has been modified from previous lots of PE-conjugated DTA-1 antibody.

References

Shimizu J, Yamazaki S, Takahashi T, Ishida Y, Sakaguchi S. Stimulation of CD25(+)CD4(+) regulatory T cells through GITR breaks immunological self-tolerance. Nat Immunol. 2002 Feb;3(2):135-42.

Related Products

Cat. 12-4031  Phycoerythrin (PE) Rat IgG2b Isotype Control
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Cat. 72-5775  PE anti-mouse/rat Foxp3 Staining Set (clone FJK-16s Set)