Product Information

Contents: Phycoerythrin (PE) anti-mouse GITR (TNFRSF18)
Catalog Number: 12-5874
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DTA-1
Isotype: Rat IgG2b

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-5874-80	25 ug	$115
12-5874-82	100 ug	$305

*International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with APC anti-mouse CD25 (PC61.5) (cat. 17-0251) and 0.06 μg of PE Rat IgG2b Isotype Control (cat. 12-4031) (left) or 0.002 μg of PE anti-mouse GITR (DTA-1) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5874	Fluorescein isothiocyanate (FITC) anti-mouse GITR (TNFRSF18, AITR)	488	518	FC
12-5874	Phycoerythrin (PE) anti-mouse GITR (TNFRSF18, AITR)	488	575	FC
13-5874	Biotin anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FC
14-5874	Affinity Purified anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FA FC IP
16-5874	Functional Grade* Purified anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FA FC
17-5874	Allophycocyanin (APC) anti-mouse GITR (TNFRSF18, AITR)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naïve mice, GITR is expressed predominantly by CD4⁺CD25⁺ T regulatory cells (Treg) and by CD25⁺ CD4⁺ CD8^- thymocytes. Stimulation of GITR abrogates Treg cell-mediated suppression. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.

Usage

For research use only, not for diagnostic or therapeutic use. The DTA-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.004 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Note: The concentration suggested above has been modified from previous lots of PE-conjugated DTA-1 antibody.

Related Products

Cat. 12-4031    Phycoerythrin (PE) Rat IgG2b Isotype Control
Cat. 71-5775    FITC anti-mouse/rat Foxp3 Staining Set (clone FJK-16s Set)
Cat. 72-5775    PE anti-mouse/rat Foxp3 Staining Set (clone FJK-16s Set)
Cat. 13-5874    Biotin anti-mouse GITR (TNFRSF18, AITR) (clone DTA-1)
Cat. 14-5874    Affinity Purified anti-mouse GITR (TNFRSF18, AITR) (clone DTA-1)

References

Shimizu J, Yamazaki S, Takahashi T, Ishida Y, Sakaguchi S. Stimulation of CD25(+)CD4(+) regulatory T cells through GITR breaks immunological self-tolerance. Nat Immunol. 2002 Feb;3(2):135-42.