Product Information

Contents: Phycoerythrin (PE) anti-mouse NKG2D / CD314 (blocking)
Catalog Number: 12-5873
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: C7
Isotype: Armenian Hamster IgG

Staining of BALB/c splenocytes with APC anti-mouse pan-NK (DX5) (cat. 17-5971) and 0.5 μg of PE Golden Syrian Hamster IgG Iso Cntrl (cat. 12-4915) (left) or 0.5μg of PE C7 (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-5873	PE anti-mouse NKG2D / CD314 (blocking)	488	575	FC
13-5873	Biotin anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FC
14-5873	Affinity Purified anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
16-5873	Functional Grade* Purified anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The C7 monoclonal antibody reacts with the mouse NKG2D. NKG2D is a lectin-like molecule expressed on both human and mouse NK cell lineage. Mouse NKG2D binds to retinoic acid-inducible RAE1-α, -β, -γ, -δ, and -ε and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1⁺ thymocytes, in vitro activated LAK cells and a subset of splenic NKT cells. A10 and C7 antibodies detect NK cells from all inbred strains of mouse tested so far. C7 is reported to interfere with the interaction of NKG2D with its ligands as shown by inhibition of lysis of Ba/F3-RAE1d by C57BL/6 LAK cells in the presence of the C7 mAb. C7 and another hamster anti-mouse NKG2D (clone A10, cat.14-5872) compete with each other for binding to transfected cells by flow cytometric analysis, suggesting that they may bind to similar epitopes or block each other by steric hindrance. C7 (neutralizing) and A10 (activating) also exhibit different functional properties.

Expression of the NKG2D antigen on mouse peripheral NK and NKT cells can be detected by flow cytometric analysis using mAb CX5 with much brighter intensity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. C7 has been reported for use in flow cytometric analysis.

Applications Tested

The C7 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ho EL, Carayannopoulos LN, Poursine-Laurent J, Kinder J, Plougastel B, Smith HR, Yokoyama WM. 2002. Costimulation of multiple NK cell activation receptors by NKG2D. J Immunol. 169(7):3667-75.

Related Products

Cat. 12-4888    Phycoerythrin (PE) Armenian Hamster IgG Isotype Control (clone eBio299Arm)
Cat. 14-5872    Affinity Purified anti-mouse NKG2D / CD314 (activating) (clone A10)
Cat. 16-5872    Functional Grade Purified anti-mouse NKG2D / CD314 (activating) (clone A10)
Cat. 12-5882    PE anti-mouse NKG2D / CD314 (blocking) (clone CX5)
Cat. 13-5882    Biotin anti-mouse NKG2D / CD314 (blocking) (clone CX5)
Cat. 14-5882    Affinity Purified anti-mouse NKG2D / CD314 (blocking) (clone CX5)
Cat. 16-5882    Functional Grade Purified anti-mouse NKG2D / CD314 (blocking) (clone CX5)
Cat. 17-5882    APC anti-mouse NKG2D / CD314 (blocking) (clone CX5)