Product Information

Contents: Phycoerythrin (PE) anti-mouse GITRL (GITR Ligand)
Catalog Number: 12-5854
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioYGL386 (YGL 386, YGL-386)
Isotype: Rat IgG1

Staining of mouse peritoneal exudate cells with 0.5 μg of PE Rat IgG1 Iso Cntrl (cat. 12-4301) (open histogram) or 0.5 μg of PE anti-mouse GITRL (eBioYGL386) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5854	Affinity Purified anti-mouse GITRL (GITR Ligand)	N/A	N/A	FC IH/F
13-5854	Biotin anti-mouse GITRL (GITR Ligand)	N/A	N/A	FC IH/F
12-5854	Phycoerythrin (PE) anti-mouse GITRL (GITR Ligand)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioYGL386 monoclonal antibody reacts with mouse GITR Ligand, also known as TL6. GITRL is a type II transmembrane protein of the TNF superfamily and is expressed by endothelial cells and peripheral blood monocytes. Interaction of GITR, expressed by CD4+CD25+ Regulatory T cells, with GITRL is important for cross-talk between T lymphocytes and endothelial cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioYGL386 (eBioYGL 386, eBioYGL-386, YGL386, YGL-386) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested

This eBioYGL386 (YGL 386, YGL-386) antibody has been tested by flow cytometric analysis of mouse peritoneal exudate cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Tone, M., Y. Tone, E. Adams, S.F. Yates, M.R. Frewin, S.P. Cobbold and H. Waldmann. 2003. Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells. Proc. Natl. Acad. Sci. USA. 100: 15059-15064. (eBioYGL386, FC, PubMed)

Related Products

Cat. 12-4301 PE Rat IgG1 Isotype Control