Product Information

Contents: Phycoerythrin (PE) anti-mouse/human OCT3/4, Pou5f1, transcription factor 1
Catalog Number: 12-5841
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: EM92
Isotype: Rat IgG2a, κ

Staining of F9 embryonal carcinoma cells with 0.25 μg of PE Rat IgG2a Iso Cntrl (cat. 12-4321) (open histogram) or 0.25 μg of PE anti-mouse OCT3/4 (EM92) (colored histogram). Total cells were used for analysis.

Staining was carried out using the eBioscience Foxp3 Staining Buffer Set (00-5523).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5841	Affinity Purified anti-mouse/human OCT3/4, Pou5f1, transcription factor 1	N/A	N/A	MIC WB
12-5841	Phycoerythrin (PE) anti-mouse/human OCT3/4, Pou5f1, transcription factor 1	488	575	IC Flow
51-5841	Alexa Fluor® 647 anti-mouse/human OCT3/4, Pou5f1, transcription factor 1	633	668	IC Flow MIC
53-5841	Alexa Fluor® 488 anti-mouse/human OCT3/4, Pou5f1, transcription factor 1	488	519	IC Flow MIC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The EM92 monoclonal antibody reacts with mouse and human Oct-3/4, encoded by the Pou5F1 gene. Oct-3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct-3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct-3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct-3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct-3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This EM92 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This EM92 antibody has been tested by intracellular staining and flow cytometric analysis of F9 embryonal carcinoma cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Staining should be carried out using the eBioscience Foxp3 Staining Buffer Set (00-5523).

References

Okamoto K, Okazawa H, Okuda A, Sakai M, Muramatsu M, Hamada H. A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell. 1990 Feb 9;60(3):461-72.

Pikarsky E, Sharir H, Ben-Shushan E, Bergman Y. Retinoic acid represses Oct-3/4 gene expression through several retinoic acid-responsive elements located in the promoter-enhancer region. Mol Cell Biol. 1994 Feb;14(2):1026-38.

Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007 Nov 30;131(5):861-72

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Cat. 12-4321 PE Rat IgG2a Isotype Control