Contents: Phycoerythrin (PE) anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)
Catalog Number: 12-5821
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: Avas12a1
Isotype: Rat IgG2a, κ
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Surface staining of bEnd.3 cells with anti-mouse Flk-1 (Avas12a1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-5821 |
Affinity Purified anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) |
N/A |
N/A |
FC
IHC
WB
|
| 13-5821 |
Biotin anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) |
N/A |
N/A |
FC
|
| 12-5821 |
PE anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) |
488 |
575 |
FC
|
| 17-5821 |
Allophycocyanin (APC) anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) |
633 |
660 |
FC
|
| 56-5821 |
Alexa Fluor® 700 anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-5821 |
Pacific Blue® anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) |
402 |
421 |
FC
|
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The Avas12a1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Kataoka, H., N. Takakura, et al. (1997)."Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells."
Dev Growth Differ 39(6): 729-40.
Related Products
Cat. 12-4321 PE Rat IgG2a Isotype Control
Mouse Cellular Antigens Chart