Product Information

Contents: Phycoerythrin (PE) anti-mouse V beta 12b TCR (Vβ12^b-TCR, Vβ-12^b TCR)
Catalog Number: 12-5816
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CTVB12b
Isotype: Rat IgG2a

Staining of C57Bl/6 splenocytes with APC anti-mouse CD3e (145-2C11) (cat. 17-0031) and 0.25 μg of PE Rat IgG2a Iso Cntrl (cat. 12-4321) (left) or 0.25 μg of PE anti-mouse Vb-12b TCR (CTVB12b) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-5816	Biotin anti-mouse V beta 12b TCR (Vb12b-TCR, Vb-12b TCR)	N/A	N/A	FC
11-5816	Fluorescein isothiocyanate (FITC) anti-mouse V beta 12b TCR (Vb12b-TCR, Vb-12b TCR)	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The CTVB12b monoclonal antibody reacts with T cell receptors (TCR) which contain Vβ 12^b.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This CTVB12b antibody has been reported for use in flow cytometric analysis.

Applications Tested

This CTVB12b antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 12-4321 PE Rat IgG2a Isotype Control