Contents: Phycoerythrin (PE) anti-mouse V beta 8.1, 8.2 TCR (Vβ8.1, 8.2-TCR, Vβ-8.1, 8.2 TCR)
Catalog Number: 12-5813
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: KJ16 (KJ-16, KJI6, KJ-I6)
Isotype: Rat IgG1
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of C57Bl/6 lymph node cells with FITC anti-mouse CD3e (145-2C11) (cat. 11-0031) and 0.25 μg of PE Rat IgG1 Iso Cntrl (cat. 12-4301) (left) or 0.25 μg of PE anti-mouse Vb8.1, 8.2 TCR (KJ16) (right). Cells in the lymphocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-5813 |
Affinity Purified anti-mouse V beta 8.1, 8.2 TCR (Vb8.1, 8.2-TCR, Vb-8.1, 8.2 TCR) |
N/A |
N/A |
FC
IP
|
| 13-5813 |
Biotin anti-mouse V beta 8.1, 8.2 TCR (Vb8.1, 8.2-TCR, Vb-8.1, 8.2 TCR) |
N/A |
N/A |
FC
IP
|
| 11-5813 |
Fluorescein isothiocyanate (FITC) anti-mouse V beta 8.1, 8.2 TCR (Vb8.1, 8.2-TCR, Vb-8.1, 8.2 TCR) |
488 |
518 |
FC
|
| 12-5813 |
Phycoerythrin (PE) anti-mouse V beta 8.1, 8.2 TCR (Vb8.1, 8.2-TCR, Vb-8.1, 8.2 TCR) |
488 |
575 |
FC
|
| 15-5813 |
Phycoerythrin-Cy5 (PE-Cy5) anti-mouse V beta 8.1, 8.2 TCR (Vb8.1, 8.2-TCR, Vb-8.1, 8.2 TCR) |
488 |
670 |
FC
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The KJ16 monoclonal antibody recognizes
mouse T cell receptors (TCR) containing Vβ 8.1 or 8.2, which is expressed by 15-
20% of peripheral T cells in most strains of mice (ie. Balb/c, AKR,
C57BL/6, B10.A) and by a number of T cell hybridomas (ie. DO-
11.10, 3DT-52.5, D1G10G11, SKK-9.11, SKK-45.10, MDK16, S18.4).
Applications Reported
For research use only, not for diagnostic or therapeutic use. This KJ16 (KJ-16, KJI6, KJ-I6) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This KJ16 (KJ-16, KJI6, KJ-I6) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Haskins, K., C. Hannum, J. White , N. Roehm, R. Kubo, J.
Kappler, and P. Marrack. 1984. The antigen-specific, major
histocompatibility complex-restricted receptor on T cells. J. Exp.
Med. 160: 452-471.
Yagüe, J., J. White, C. Coleclongh, J. Kappler, E. Palmer and
P. Marrack. 1985. The T cell receptor: the γ and ß chains define
idiotype, and antigen and MHC specificity. Cell 42: 81-87.
Sim, G. K., A. A. Augstin. 1985. Vß gene polymorhism and a
major polyclonal T cell receptor idiotype. Cell 42: 89-92.
Related Products
Cat.
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Mouse Cellular Antigens Chart