Product Information

Contents: Phycoerythrin (PE) anti-mouse V alpha 2 TCR (Vα2-TCR, Vα-2 TCR)
Catalog Number: 12-5812
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: B20.1
Isotype: Rat IgG2a

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5812	Affinity Purified anti-mouse V alpha 2 TCR (Va2-TCR, Va-2 TCR)	N/A	N/A	FC IP
13-5812	Biotin anti-mouse V alpha 2 TCR (Va2-TCR, Va-2 TCR)	N/A	N/A	FC IP
11-5812	Fluorescein isothiocyanate (FITC) anti-mouse V alpha 2 TCR (Va2-TCR, Va-2 TCR)	488	518	FC
12-5812	Phycoerythrin (PE) anti-mouse V alpha 2 TCR (Va2-TCR, Va-2 TCR)	488	575	FC
17-5812	Allophycocyanin (APC) anti-mouse V alpha 2 TCR (Va2-TCR, Va-2 TCR)	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) Vα-2 chains. This antibody recognizes the majority of the TCR Vα-2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, Vδ8 due to the high degree of homology.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This B20.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended to preblock Fc receptors for 10 minutes using 0.5 µg of purified anti-mouse CD16/32 (cat. 14-0161). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Pircher, H., N. Rebai, M. Groettrup, C. Gregoire, D. E. Speiser, M. P. Happ, E. Palmer, R. M. Zinkernagel, H. Hengartner, B. Malissen. 1992. Eur. J. Immunol. 22: 399-404.

Gregoire, C., N. Rebai, F. Schweisguth, A. Necker, G. Mazza, N. Auphan, A. Millward, Anne-Marie Schmitt-Verhulst, B. Malissen. 1991. Proc. Natl. Acad. Sci. USA. 88: 8077-8081.

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