Product Information

Contents: Phycoerythrin (PE) anti-human V alpha 24 J alpha 18 TCR (Va24-Ja18 TCR)
Catalog Number: 12-5806
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 6B11
Isotype: Mouse IgG1, k

Staining of normal human peripheral blood mononuclear cells with FITC anti-human CD3 (UCHT1) (cat. 11-0038) and PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or PE anti-human V alpha 24 J alpha 18 TCR (6B11) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5806	Affinity Purified anti-human V alpha 24 J alpha 18 TCR (Va24-Ja18 TCR)	N/A	N/A	FC
12-5806	Phycoerythrin (PE) anti-human V alpha 24 J alpha 18 TCR (Va24-Ja18 TCR)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 6B11 monoclonal antibody reacts with an epitope of the CDR3 formed by the germ-line configuration of the Vα24 and Jα18 of the TCR α locus, which results in the expression of an invariant T-cell receptor (TCR) expressed by invariant NK T cells (iNKT). This TCR is homologous to the murine Vα14Jα18. This TCR α chain pairs with a restricted set of rearranged TCR β chains, with Vβ11 being the most prominent in humans. NK T cells are restricted by the CD1d molecule and are activated by a CD1d-presented glycolipid ligand, which results in the rapid production of IL-4, IL-13 and IFNγ. Although iNKT cells have NK-like cytolytic activity, they are considered regulators of immune responses because they rapidly produce large amounts of both Th1 and Th2 cytokines in autoimmune disease, infectious disease, and cancer. The number of iNKT cells present in peripheral blood of normal humans shows considerable variability ranging from 0.01-1% of total peripheral T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 6B11 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 6B11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood mononuclear cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Montoya CJ, Pollard D, Martinson J, Kumari K, Wasserfall C, Mulder CB, Rugeles MT, Atkinson MA, Landay AL, Wilson SB.Characterization of human invariant natural killer T subsets in health and disease using a novel invariant natural killer T cell-clonotypic monoclonal antibody, 6B11.Immunology. 2007 Sep;122(1):1-14. (6B11, mAb characterization, PubMed)

Illés Z, Kondo T, Newcombe J, Oka N, Tabira T, Yamamura T.Differential expression of NK T cell V alpha 24J alpha Q invariant TCR chain in the lesions of multiple sclerosis and chronic inflammatory demyelinating polyneuropathy.J Immunol. 2000 Apr 15;164(8):4375-81.

Thomas SY, Hou R, Boyson JE, Means TK, Hess C, Olson DP, Strominger JL, Brenner MB, Gumperz JE, Wilson SB, Luster AD.CD1d-restricted NKT cells express a chemokine receptor profile indicative of Th1-type inflammatory homing cells.J Immunol. 2003 Sep 1;171(5):2571-80. (6B11, FC, PubMed)

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Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control