Product Information

Contents: Phycoerythrin (PE) anti-mouse IgM
Catalog Number: 12-5790
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: II/41
Isotype: Rat IgG2a, κ

Staining of BALB/c bone marrow cells with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.06 μg of PE Rat IgG2a Iso Cntrl (cat. 12-4321) (left) or 0.06 μg of PE II/41 (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5790	Affinity Purified anti-mouse IgM	N/A	N/A	ELISA FC IH/F
13-5790	Biotin anti-mouse IgM	N/A	N/A	FC
11-5790	Fluorescein (FITC) anti-mouse IgM	488	518	FC
12-5790	Phycoerythrin (PE) anti-mouse IgM	488	575	FC
15-5790	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse IgM	488	670	FC
17-5790	Allophycocyanin (APC) anti-mouse IgM	633	660	FC
25-5790	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IgM	488	760	FC
35-5790	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse IgM	488	690	FC
36-5790	Functional Grade* Biotin anti-mouse IgM	N/A	N/A	FC
95-5790	eFluor^TM 650^NC anti-mouse IgM	350-500	650	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The II/41 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. 1993. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 150(12):5252-62.

Related Products

Cat. 12-4321  PE Rat IgG2a Isotype Control
Cat. 11-5890  FITC anti-mouse IgM (clone eB121-15F9)
Cat. 12-5890  PE anti-mouse IgM (clone eB121-15F9)
Cat. 13-5890  Biotin anti-mouse IgM (clone eB121-15F9)
Cat. 14-5890  Affinity Purified anti-mouse IgM (clone eB121-15F9)