PE anti-human MICA/MICB (MIC A/B) antibody: r-phycoerythin (r-PE) conjugate

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Contents: Phycoerythrin (PE) anti-human MICA/MICB (MIC A/B)
Catalog Number: 12-5788
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 6D4
Isotype: Mouse IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-5788-71	25 tests	$100
12-5788-73	100 tests	$220

*International customers: Please contact your distributor for region specific pricing.

Staining of HeLa cells with PE Mouse IgG2a, K Iso Cntrl (cat. 12-4724) (open histogram) or PE anti-human MICA/MICB (6D4) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5788	Affinity Purified anti-human MICA/MICB (MIC A/B)	N/A	N/A	FA FC IHC IP
16-5788	Functional Grade* Purified anti-human MICA/MICB (MIC A/B)	N/A	N/A	FA FC IHC IP
12-5788	Phycoerythrin (PE) anti-human MICA/MICB (MIC A/B)	488	575	FC
53-5788	Alexa Fluor® 488 anti-human MICA/MICB (MIC A/B)	488	519	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind β2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, γδ T cells and αβ CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 6D4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 6D4 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cells. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Groh V, Steinle A, Bauer S, Spies T. Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells. Science. 1998 Mar 13;279(5357):1737-40. (6D4, FA, FC, PubMed)

Li Z, Groh V, Strong RK, Spies T. A single amino acid substitution causes loss of expression of a MICA allele. Immunogenetics. 2000 Mar;51(3):246-8. (6D4, FC, WB, PubMed)

Jinushi M, Takehara T, Kanto T, Tatsumi T, Groh V, Spies T, Miyagi T, Suzuki T, Sasaki Y, Hayashi N. Critical role of MHC class I-related chain A and B expression on IFN-alpha-stimulated dendritic cells in NK cell activation: impairment in chronic hepatitis C virus infection. J Immunol. 2003 Feb 1;170(3):1249-56. (6D4, FC, FA, PubMed)

Related Products

Cat. 12-4724 Phycoerythrin (PE) Mouse IgG2a, K Isotype Control
Cat. 13-5878 Biotin anti-human NKG2D / CD314 (activating) (clone 1D11)