PE anti-human p75/AIRM1 (p75, AIRM1, QA79, Siglec-7) antibody: r-phycoerythin (r-PE) conjugate

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Contents: Phycoerythrin (PE) Human p75/AIRM1
Catalog Number: 12-5759
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioQA79 (QA79)
Isotype: Mouse IgG1

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-5759-71	25 tests	$150
12-5759-73	100 tests	$290

*International customers: Please contact your distributor for region specific pricing.

Left: Staining of normal human peripheral blood cells with FITC anti-human CD56 (MEM188) (cat. 11-0569) and PE Human p75/AIRM1. Cells in the lymphocyte gate were used for analysis. Right: Staining of normal human peripheral blood cells with 0.25μg of mIgG1 PE Isotype control (open histogram) and 0.25μg of PE anti-human p75/AIRM1 (QA79) (colored histogram). Cells in the monocyte gate were used for analysis

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5759	Affinity Purified anti-human p75/AIRM1 (p75, AIRM1, QA79, Siglec-7)	N/A	N/A	FC IP
13-5759	Biotin anti-human p75/AIRM1 (p75, AIRM1, QA79, Siglec-7)	N/A	N/A	FC
12-5759	Phycoerythrin (PE) anti-human p75/AIRM1 (p75, AIRM1, QA79, Siglec-7)	488	575	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioQA79 monoclonal antibody reacts with adhesion inhibitory receptor molecule 1 (AIRM1), also known as sialic acid binding immunoglobulin-like lectin 7 (Siglec-7), p75 and CDw328. AIRM1 is a cell surface receptor that belongs to the sialoadhesin family and displays homology with the myeloid cell antigen CD33. p75/AIRM1 expression is confined to NK cells in lymphoid cells. eBioQA79 crosslinking inhibits NK function. p75/AIRM1 is expressed at various stages of myeloid differentiation. Presence of this protein is a useful way to monitor cellular proliferation and determine proliferative states of cells, especially in diseases such as chronic myeloid leukemia.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioQA79 (QA79) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioQA79 (QA79) antibody has been pre-titrated and tested by flow cytometric analysis. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Falco M, Biassoni R, Bottino C, Vitale M, Sivori S, Augugliaro R, Moretta L, Moretta A. Identification and molecular cloning of p75/AIRM1, a novel member of the sialoadhesin family that functions as an inhibitory receptor in human natural killer cells. J Exp Med. 1999 Sep 20;190(6):793-802.

Vitale C, Romagnani C, Puccetti A, Olive D, Costello R, Chiossone L, Pitto A, Bacigalupo A, Moretta L, Mingari MC. Surface expression and function of p75/AIRM-1 or CD33 in acute myeloid leukemias: engagement of CD33 induces apoptosis of leukemic cells. Proc Natl Acad Sci U S A. 2001 May 8;98(10):5764-9. (FC, PubMed)

Vitale C, Romagnani C, Falco M, Ponte M, Vitale M, Moretta A, Bacigalupo A, Moretta L, Mingari MC. Engagement of p75/AIRM1 or CD33 inhibits the proliferation of normal or leukemic myeloid cells. Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15091-6. (FC, PubMed)

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