Staining of dendritic cell line with 0.125 µg of Mouse IgG1 κ Isotype Control PE (cat. 12-4714) (open histogram) or 0.125 µg of Anti-Mouse RAE1δ PE (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse RAE1 delta PE Catalog Number: 12-5756 Concentration: 0.2 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: RD-41 Host/Isotype: Mouse IgG1
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Size
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12-5756-81
50 ug
12-5756-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The RD-41 monoclonal antibody reacts with the murine Rae1δ. Rae-1δ is one of several known murine NKG2D ligands that include RAE-1 molecules (α, β, ε, γ and δ), H60 and MULT-1. Expression of NKG2D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2D ligands which in turn activates NK cells tumoricidal activity through NKG2D. Until now, the expression of NKG2D ligands has been mainly studied with NKG2D tetramers which recognizes all NKG2D ligands. The RD-41 antibody has been reported to block tetramer staining.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This RD-41 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This RD-41 antibody has been tested by flow cytometric analysis on dendritic cell line. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Diefenbach A, Jamieson AM, Liu SD, Shastri N, Raulet DH.
Ligands for the murine NKG2D receptor: expression by tumor cells and activation of NK cells and macrophages.
Nat Immunol. 2000 Aug;1(2):119-26.
Cerwenka A, Bakker AB, McClanahan T, Wagner J, Wu J, Phillips JH, Lanier LL.
Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice.
Immunity. 2000 Jun;12(6):721-7.
O'Callaghan CA, Cerwenka A, Willcox BE, Lanier LL, Bjorkman PJ.
Molecular competition for NKG2D: H60 and RAE1 compete unequally for NKG2D with dominance of H60.
Immunity. 2001 Aug;15(2):201-11.
Diefenbach A, Jensen ER, Jamieson AM, Raulet DH.
Rae1 and H60 ligands of the NKG2D receptor stimulate tumour immunity.
Nature. 2001 Sep 13;413(6852):165-71.
Carayannopoulos LN, Naidenko OV, Kinder J, Ho EL, Fremont DH, Yokoyama WM.
Ligands for murine NKG2D display heterogeneous binding behavior.
Eur J Immunol. 2002 Mar;32(3):597-605.
