Product Information

Contents: Phycoerythrin (PE) anti-mouse Plexin-B2
Catalog Number: 12-5665
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio3E7 (3E7)
Isotype: Armenian Hamster IgG

(Left): Staining of PDV cells with 0.125 μg of PE Armenian Hamster IgG Iso Cntrl (cat. 12-4888) (open histogram) or 0.125 μg of PE anti-mouse Plexin-B2 (colored histogram). Total viable cells were used for analysis.

(Right): Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.125 μg of PE anti-mouse Plexin-B2 (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5665	Affinity Purified anti-mouse Plexin-B2	N/A	N/A	FC IHC IP
13-5665	Biotin anti-mouse Plexin-B2	N/A	N/A	FC
12-5665	Phycoerythrin (PE) anti-mouse Plexin-B2	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

Plexin-B2 is a member of the Semaphorin receptor family. The semaphorin family consists of membrane and secreted proteins involved in attraction and repulsion of neuronal guidance cues. Plexin-B1 is a transmembrane protein that can be cleaved coverting the single chain presursor into a heterodimeric receptor. It has been shown to bind Sema4D/CD100 and is involved in axon development in mouse embryos. Sema4D also plays a role in the immune system interacting with CD72. Plexin-B2's possible role in the immune system has not been characterized.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio3E7 (3E7) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio3E7 (3E7) antibody has been tested by flow cytometric analysis mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Artigiani S, Barberis D, Fazzari P, Longati P, Angelini P, van de Loo JW, Comoglio PM, Tamagnone L. Functional regulation of semaphorin receptors by proprotein convertases. J Biol Chem. 2003 Mar 21;278(12):10094-101. (PubMed)

Raper JA. Semaphorins and their receptors in vertebrates and invertebrates. Curr Opin Neurobiol. 2000 Feb;10(1):88-94. (PubMed)

Worzfeld T, Puschel AW, Offermanns S, Kuner R. Plexin-B family members demonstrate non-redundant expression patterns in the developing mouse nervous system: an anatomical basis for morphogenetic effects of Sema4D during development. Eur J Neurosci. 2004 May;19(10):2622-32. (PubMed)

Related Products

Cat. 12-4888 Phycoerythrin (PE) Armenian Hamster IgG Isotype Control (clone eBio299Arm)