Product Information

Contents: Phycoerythrin (PE) anti-mouse Galectin-3 / Mac-2
Catalog Number: 12-5301
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioM3/38 (M3/38)
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 bone marrow cells with FITC anti-mouse/human CD11b (M1/70) (cat. 11-0112) and 0.25 μg of PE Rat IgG2a Iso Cntrl (cat. 12-4321) (left) or 0.03 μg of PE anti-mouse Galectin-3 (M3/38) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5301	Affinity Purified anti-mouse Galectin-3 / Mac-2	N/A	N/A	ELISA FC IH/F IHC(Paraffin) IP WB
13-5301	Biotin anti-mouse Galectin-3 / Mac-2	N/A	N/A	FC
12-5301	Phycoerythrin (PE) anti-mouse Galectin-3 / Mac-2	488	575	FC
51-5301	Alexa Fluor® 647 anti-mouse Galectin-3 / Mac-2	633	668	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.

This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse thioglycolate elicited peritoneal macrophages. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ho MK, Springer TA. 1982. MAC-2, A Novel 32000 Mr Mouse Macrophage Subpopulation-Specific Antigen Defined by Monoclonal Antibodies. J. Immunol. 128(3):1221-1228.

Joo G-H, Goedegebuure PS, Sadanaga N, Nagoshi M, von Bernstorff W, Eberlein TJ. Expression and function of galectin-3, a b-galactoside-binding protein in activated T lymphocytes J. Leukoc. Biol. 69: 555–564; 2001.

Oliveira FL, Fraza P, Chammas R, Hsu DK, Liu FT, Borojevic R, Takiya CM, El-Cheikh MC, Kinetics of mobilization and differentiation of lymphohematopoietic cells during experimental murine schistosomiasis in galectin-3-/- mice J. Leukoc. Biol. 82: ePub; 2007

Springer TA, 1981. Monoclonal Antibody Analysis of Complex Biological Systems. J. Biol Chem. 256(8):3833-3839.

Related Products

Cat. 12-4321 PE Rat IgG2a Isotype Control