Product Information

Contents: Phycoerythrin (PE) anti- human ILT7, novel pDC marker
Catalog Number: 12-5179
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio17G10.2 (17G10.2)
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with PE-Cy7 anti-human CD123 (6H6) (cat. 25-1239) and PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or PE anti- human ILT7, novel pDC marker (right). Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
16-5179	Functional Grade* Purified anti-human ILT7 (novel pDC marker, CD85g, IL-T7)	N/A	N/A	FC
12-5179	Phycoerythrin (PE) anti-human ILT7 (novel pDC marker, CD85g, IL-T7)	488	575	FC
51-5179	Alexa Fluor® 647 anti-human ILT7 (novel pDC marker, CD85g, IL-T7)	633	668	FC
45-5179	PerCP-Cy5.5 anti-human ILT7 (novel pDC marker, CD85g, IL-T7) (PE-Cy5.5 replacement)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio17G10.2 monoclonal antibody reacts with human immunoglobulin-like transcript 7 (ILT7), also known as CD85g. ILT7 is part of the leukocyte immunoglobulin-like receptor (LIR) gene family and associates with FcεR1γ to form a functional complex which transduces ITAM-mediated signals that negatively modulates TLR-induced type I IFN production by human plasmacytoid dendritic cells. ILT7 is expressed exclusively on plasmacytoid dendritic cells and is absent in monocyte derived DCs. Treatment with IL-3 or CpG causes a decrease in expression.

Double staining of human PBMCs with eBio17G10.2 and anti-pDC marker BDCA2 yields virtually complete co-staining. Cross linking of ILT7 with eBio17G10.2 antibody causes reduced transcription of IFN-α1, IFN-α2, IFN-α4 and IFN-β.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio17G10.2 (17G10.2) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio17G10.2 (17G10.2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Cao, W., et al. 2006. Plasmacytoid dendritic cell–specific receptor ILT7–FcRI inhibits Toll-like receptor–induced interferon production. J. Exp. Med. 203: 1399-1405.

Ju XS, Hacker C, Scherer B, Redecke V, Berger T, Schuler G, Wagner H, Lipford GB, Zenke M. Immunoglobulin-like transcripts ILT2, ILT3 and ILT7 are expressed by human dendritic cells and down-regulated following activation. Gene. 2004 Apr 28;331:159-64.

Rissoan MC, Duhen T, Bridon JM, Bendriss-Vermare N, Peronne C, de Saint Vis B, Briere F, Bates EE. Subtractive hybridization reveals the expression of immunoglobulin-like transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells. Blood. 2002 Nov 1;100(9):3295-303.

Related Products

Cat. 25-1239 Phycoerythrin-Cy7 (PE-Cy7) anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra) (clone 6H6)
Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control