Product Information

Contents: Phycoerythrin (PE) anti-human ILT5 (CD85a, LIR3)
Catalog Number: 12-5159
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MKT5.7H5.1
Isotype: Rat IgG2a, κ

Staining of normal human peripheral blood cells with 0.25 μg of PE Rat IgG2a Iso Cntrl (cat. 12-4321) (open histogram) or PE anti-human ILT5 (MKT5.7H5.1) (colored histogram). Cells in the monocyte (left) and granulocyte (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-5159	Phycoerythrin (PE) anti-human ILT5 (CD85a, LIR3)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody MKT5.7H5.1 recognizes ILT5 also known as CD85a. ILT5 is a member of the ILT (immunoglobulin-like transcript)/ LIR (leuokocyte Ig-like receptor) MIR (monocytes Ig-like receptor) family. It is a transmembrane protein that contains 4 extracellular Ig domains and 4 ITIMs. Expression is found on monocytes, DC and granulocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MKT5.7H5.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This MKT5.7H5.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

References

Colonna M, Samaridis J, Cella M, Angman L, Allen RL, O'Callaghan CA, Dunbar R, Ogg GS, Cerundolo V, Rolink A. Human myelomonocytic cells express an inhibitory receptor for classical and nonclassical MHC class I molecules. J Immunol. 1998 Apr 1;160(7):3096-100.

Related Products

Cat. 12-4321 PE Rat IgG2a Isotype Control