Product Information

Contents: Phycoerythrin (PE) anti-human ILT3 (GM 4d, CD85k)
Catalog Number: 12-5139
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: ZM4.1
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with 0.25 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or PE anti-human ILT3 (ZM4.1) (colored histogram)(left). Evaluation of pDC expression by costaining PerCP-Cy5.5 CD123 (45-1239) and PE anti-human ILT3 (ZM4.1). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
16-5139	Functional Grade* Purified anti-human ILT3 (GM 4d, CD85k)	N/A	N/A	FA FC
12-5139	Phycoerythrin (PE) anti-human ILT3 (GM 4d, CD85k)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody ZM4.1 recognizes ILT3, also known as CD85k. ILT3 is a member of the large family of ILT molecules (Immunologulin-like Transcripts, Leucocyte Ig-like Receptors (LIRs) and Monocyte/Macrophage Ig-like Receptors (MIRs). ILT3 is a 60kDa transmembrane protein with two Ig domains and ITIMs. Expression is found on monocytes, macrophages, and dendritic cells. High levels of ILT3 expression is also found on plasmacytoid dendritic cells (pDCs). ILT3 expression by DCs has been shown to be required to induce Regulatory Tcells (tregs). Addition of ZM4.1, like ZM3.8, blocks the suppressive function of the Tregs in in vitro culture systems with allogenic DC’s.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This ZM4.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This ZM4.1 antibody has been pre-titrated and tested flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

References

Cella M, Döhring C, Samaridis J, Dessing M, Brockhaus M, Lanzavecchia A, Colonna M. A novel inhibitory receptor (ILT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen processing. J Exp Med. 1997 May 19;185(10):1743-51. (ILT3, IP, FC, PubMed)

Penna G, Roncari A, Amuchastegui S, Daniel KC, Berti E, Colonna M, Adorini L. Expression of the inhibitory receptor ILT3 on dendritic cells is dispensable for induction of CD4+Foxp3+ regulatory T cells by 1,25-dihydroxyvitamin D3. Blood. 2005 Nov 15;106(10):3490-7. (ILT3, FA, PubMed)

Related Products

Cat. 17-1239    Allophycocyanin (APC) anti-human CD123 (Interleukin-3 Receptor alpha, IL-3 Receptor alpha, IL-3Ra, IL3Ra) (clone 6H6)
Cat. 12-4714    Phycoerythrin (PE) Mouse IgG1, K Isotype Control
Cat. 12-5119    Phycoerythrin (PE) anti-human ILT1 (CD85h, LIR7, LILRA2) (clone 135.4)
Cat. 11-5148    Fluorescein isothiocyanate (FITC) anti-human ILT4 (CD85d) blocking (clone 27D6)
Cat. 12-5159    Phycoerythrin (PE) anti-human ILT5 (CD85a, LIR3) (clone MKT5.7H5.1)