Product Information

Contents: Phycoerythrin (PE) anti-human ILT1 (CD85h, LIR7)
Catalog Number: 12-5119
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 135.4
Isotype: Rat IgG2a, k

Staining of normal human peripheral blood cells with 0.5 μg of PE Rat IgG2a Iso Cntrl (cat. 12-4321) (open histogram) or PE anti-human ILT1 (135.4) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-5119	Phycoerythrin (PE) anti-human ILT1 (CD85h, LIR7, LILRA2)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody 135.4 recognizes ILT1, also known as CD85h, LIR7 and LILRA2. ILT1 is a member of the large family of ILT molecules (Immunologulin-like Transcripts) also known as Leukocyte Ig-like Receptors (LIRs) and Monocyte/Macrophage Ig-like Receptors (MIRs). ILT1 contains a short cytoplasmic domain. Through the cytoplasmic domain ILT1 associates with the Fc receptor γ chain but not DAP12 resulting in a stimulatory signal. ILT1 may act as a receptor for MHC Class I antigens. Expression of ILT1 is mainly found on monocytes, macrophages, DC and weakly on granulocytes. ILT1 is thought to play a role in the TH1/Th2 balance as well as TLR signaling. The monoclonal antibody 135.4 has been reported to have activating properties upon crosslinking which result in calcium influx.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 135.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 135.4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

Mamegano K, Kuroki K, Miyashita R, Kusaoi M, Kobayashi S, Matsuta K, Maenaka K, Colonna M, Ozaki S, Hashimoto H, Takasaki Y, Tokunaga K, Tsuchiya N. Association of LILRA2 (ILT1, LIR7) splice site polymorphism with systemic lupus erythematosus and microscopic polyangiitis. Genes Immun. 2008 Feb 14;1-10 (135.4, FC, PubMed)

Nakajima H, Samaridis J, Angman L, Colonna M.Human myeloid cells express an activating ILT receptor (ILT1) that associates with Fc receptor gamma-chain. J Immunol. 1999 Jan 1;162(1):5-8. (135.4, FC, IP, FA PubMed)

Penna G, Roncari A, Amuchastegui S, Daniel KC, Berti E, Colonna M, Adorini L. Expression of the inhibitory receptor ILT3 on dendritic cells is dispensable for induction of CD4+Foxp3+ regulatory T cells by 1,25-dihydroxyvitamin D3. Blood. 2005 Nov 15;106(10):3490-7. (135.4, FA, PubMed)

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Cat. 12-4321 PE Rat IgG2a Isotype Control