rat IgG antibody, Phycoerythrin (PE) conjugate

Contents: PE Donkey F(ab')2 Fragment anti-Rat IgG (H+L) Minimal Reactivity to Mouse IgG
Catalog Number: 12-4822
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: polyclonal
Isotype: Donkey IgG

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12-4822-82

100 ug

12-4822-87

250 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-4822	PE Donkey F(ab')2 Fragment anti-Rat IgG (H+L) Minimal Reactivity to Mouse IgG	488	575	FC
17-4822	APC Donkey F(ab')2 Fragment anti-Rat IgG (H+L) Minimal Reactivity to Mouse IgG	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

This antibody will recognize the light chains of other rat immunoglobulins. Reactions to non-immunoglobulin rat serum proteins have not been detected.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This polyclonal antibody has been reported for use in flow cytometric analysis.

Applications Tested

This polyclonal antibody has been tested by flow cytometric analysis to detect purified rat monoclonal antibodies. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, it has been adsorbed and tested by ELISA to ensure minimal cross reaction to human, bovine, chicken, goat, guinea pig, syrian hamster, rabbit, sheep and horse serum proteins, but may cross-react with immunoglobulins from other species.