Product Information

Contents: Phycoerythrin (PE) Mouse IgM Isotype Control
Catalog Number: 12-4752
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Isotype: Mouse IgM, λ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-4752	Affinity Purified Mouse IgM Isotype Control	N/A	N/A	FC
16-4752	Functional Grade* Purified Mouse IgM Isotype Control	N/A	N/A	FC
11-4752	FITC Mouse IgM Isotype Control	488	518	FC
12-4752	PE Mouse IgM Isotype Control	488	575	FC
57-4752	Pacific Blue® Mouse IgM Isotype Control	402	421	FC
94-4752	eFluor^TM 625^NC Mouse IgM Isotype Control	350-500	625	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The mouse IgM, λ is useful as an isotype control immunoglobulin. The immunoglobulin was generated by immunization with lipopolysaccharide (LPS). The immunoglobulin is reactive with LPS.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This mouse IgM isotype control has been reported for use in flow cytometric analysis and ELISA.

Applications Tested

This mouse IgM isotype control has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes and can be used at 10 μl per million cells (100 μl blood).