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Contents: Phycoerythrin (PE) Rat IgG2a isotype control Catalog Number: 12-4321 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.1% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. Isotype: Rat IgG2a, κ Prices for This Product* Cat. No. Size Price Qty Action 12-4321-71 25 tests $85 12-4321-73 100 tests $140 12-4321-81 50 ug $85 12-4321-82 100 ug $140 12-4321-83 200 ug $250 *International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
10-4321	**May be discontinued; see superior product APC-Alexa Fluor® 750** Allophycocyanin-Cy7 (APC-Cy7) Rat IgG2a Isotype Control	633	760	FC
11-4321	FITC Rat IgG2a Isotype Control	488	518	FC
12-4321	PE Rat IgG2a Isotype Control	488	575	FC
13-4321	Biotin Rat IgG2a Isotype Control	N/A	N/A	FC
14-4321	Affinity Purified Rat IgG2a Isotype Control	N/A	N/A	FC
15-4321	PE-Cy5 Rat IgG2a Isotype Control	488	670	FC
16-4321	Functional Grade* Purified Rat IgG2a Isotype Control	N/A	N/A	FC
17-4321	APC Rat IgG2a Isotype Control	633	660	FC
25-4321	Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2a Isotype Control	488	760	FC
27-4321	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2a Isotype Control (APC-Cy7 Equivalent)	633	779	FC
30-4321	DISCONTINUED - Allophycocyanin-Cy5.5 (APC-Cy5.5) Rat IgG2a Isotype Control (Alexa Fluor® 700 Equivalent)	633	690	FC
35-4321	PE-Cy5.5 Rat IgG2a Isotype Control	488	690	FC
36-4321	Functional Grade* Biotin Rat IgG2a Isotype Control	N/A	N/A	IVCCA
45-4321	PerCP-Cy5.5 Rat IgG2a Isotype Control	488	690	FC
51-4321	Alexa Fluor® 647 Rat IgG2a Isotype Control	633	668	FC
53-4321	Alexa Fluor® 488 Rat IgG2a Isotype Control	488	519	FC
56-4321	Alexa Fluor® 700 Rat IgG2a Isotype Control (APC-Cy5.5 Equivalent)	633	723	FC
57-4321	Pacific Blue® Rat IgG2a Isotype Control	402	421	FC
72-4321	Phycoerythrin (PE) Set Rat IgG2a Isotype Control	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The monoclonal rat IgG2a, κ is useful as an isotype control immunoglobulin.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. This rat IgG2a isotype control has been reported for use in flow cytometric analysis.

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Applications Tested

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PE Rat IgG2a Iso Cntrl is offered in 2 formats:

- μg size: This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocyte suspension. This can be used at the same concentration as the experimental antibody.

- test size: has been pre-titrated and tested by flow cytometric analysis human peripheral blood cells. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume). The pre-titrated test size contains 0.2% BSA.