Product Information

Contents: Phycoerythrin (PE) Streptavidin
Catalog Number: 12-4317
Sizes: 250 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
10-4317	Streptavidin Allophycocyanin-Cy7 (SA-APC-Cy7)	633	760	FC
11-4317	Streptavidin-FITC (Fluorescein isothiocyanate)	488	518	FC
12-4317	Streptavidin-PE (Phycoerythrin)	488	575	FC
15-4317	Streptavidin-PE-Cy5 (Phycoerythrin-Cy5)	488	670	FC
17-4317	Streptavidin Allophycocyanin (SA-APC)	633	660	FC
19-4317	Streptavidin-Cy5	633	670	FC
25-4317	Streptavidin-PE-Cy7 (Phycoerythrin-Cy7)	488	760	FC
30-4317	Streptavidin-APC-Cy5.5 (Alexa Fluor® 700 Equivalent)	633	690	FC
35-4317	Streptavidin-PE-Cy5.5	488	690	FC
45-4317	Streptavidin-PerCP-Cy5.5	488	690	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Streptavidin-PE has been reported for use in flow cytometric analysis.

Applications Tested

Streptavidin-PE has been tested by flow cytometric analysis to detect biotinylated primary antibodies. This can be used at less than or equal to 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.