Product Information

Contents: Phycoerythrin (PE) anti-mouse NKp46 (CD335)
Catalog Number: 12-3351
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 29A1.4
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with FITC anti-mouse CD3e (145-2C11) (cat. 11-0031) and 0.25μg of Rat IgG2a Iso Cntrl (cat.12-4321) (left) or 0.25 μg of PE anti-mouse NKp46 (29A1.4) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-3351	Fluorescein isothiocyanate (FITC) anti-mouse NKp46 (CD335)	488	518	FC
12-3351	Phycoerythrin (PE) anti-mouse NKp46 (CD335)	488	575	FC
51-3351	Alexa Fluor® 647 anti-mouse NKp46 (CD335)	633	668	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells (as defined as DX5- CD3-) and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL. CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.

The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 29A1.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 29A1.4 antibody has been tested by flow cytometric analysis or mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Walzer T, Chiossone L, Chaix J, Calver A, Carozzo C, Garrigue-Antar L, Jacques Y, Baratin M, Tomasello E, Vivier E. Natural killer cell trafficking in vivo requires a dedicated sphingosine 1-phosphate receptor. Nat Immunol. 2007 Dec;8(12):1337-44. (29A1.4, FC PubMed)

Walzer T, Bléry M, Chaix J, Fuseri N, Chasson L, Robbins SH, Jaeger S, André P, Gauthier L, Daniel L, Chemin K, Morel Y, Dalod M, Imbert J, Pierres M, Moretta A, Romagné F, Vivier E. Identification, activation, and selective in vivo ablation of mouse NK cells via NKp46. Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3384-9. (29A1.4, FC, FA PubMed)

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Cat. 12-4321 PE Rat IgG2a Isotype Control