Contents: Phycoerythrin (PE) anti-mouse PDCA-1 (pDC Ag-1, BST2, CD317)
Catalog Number: 12-3172
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio927
Isotype: Rat IgG2b
| Prices for This Product* |
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Price |
Qty |
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*International customers: Please contact your distributor for region specific pricing.
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Staining of SJL splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and PE anti-mouse PDCA-1 (pDC Ag-1, BST2, CD317)(left) or APC anti-mouse CD11c (N418)(cat.17-0114) and PE anti-mouse PDCA-1 (pDC Ag-1, BST2, CD317) (right). Cells in the large scatter population were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-3172 |
Affinity Purified anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC) |
N/A |
N/A |
FC
|
| 16-3172 |
Functional Grade* Purified anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC) |
N/A |
N/A |
FA
FC
|
| 13-3172 |
Biotin anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC) |
N/A |
N/A |
FC
|
| 11-3172 |
Fluorescein isothiocyanate (FITC) anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC) |
488 |
518 |
FC
|
| 12-3172 |
Phycoerythrin (PE) anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC) |
488 |
575 |
FC
|
| 51-3172 |
Alexa Fluor® 647 anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC) |
633 |
668 |
FC
|
| 53-3172 |
Alexa Fluor® 488 anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC) |
488 |
519 |
FC
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naïve mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8.
The eBio927 monoclonal antibody has also been shown to have functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify and identify pDCs.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio927 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio927 antibody has been tested by flow cytometric analysis of SJL splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Blasius A.L., E. Giurisat, M. Cella, R.D. Schreiber, A.S. Shaw, M. Colonna. 2006. Bone Marrow Stromal Cell Antigen 2 Is a Specific Marker of Type I IFN-Producing Cells in the Naïve Mouse, but a Promiscuous Cell Surface Antigen Following IFN Stimulation. J Immunol. 177:3260-3265 (927, FC, FA)
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12-4031 Phycoerythrin (PE) Rat IgG2b Isotype Control
Mouse Cellular Antigens Chart