Staining of C57Bl/6 splenocytes with 0.125 μg of PE Armenian Hamster IgG Iso Cntrl (cat. 12-4888) (blue line) or 0.125 μg of PE anti-mouse LAIR-1 (113) (purple line). Total viable F4/80 positive cells were used for analysis.
Contents: Phycoerythrin (PE) anti-mouse LAIR-1 (CD305, Leukocyte-Associated Ig-Like Receptor) Catalog Number: 12-3051 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Clone: 113 Host/Isotype: Armenian Hamster IgG Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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Size
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12-3051-81
50 ug
12-3051-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
CD305 or Leukocyte-associated Ig-like receptor-1 (LAIR-1) is a 46 kD cell surface molecule that functions as an inhibitory collagen-receptor on the majority of cells of the immune system. The mouse LAIR-1 molecule is expressed primarily on monocytes/macrophages and also on T cells, NK cells and dendritic cells. Unlike human B cells , mouse B cells do not express LAIR-1. The cytoplasmic tail of mouse LAIR-1 can be phosphorylated leading to the recruitment of SH2-containing tyrosine phosphatase(SHP)-2 and C-terminal Src kinase.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 113 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 113 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Meyaard L.
The inhibitory collagen receptor LAIR-1 (CD305). J Leukoc Biol. 2008 Apr;83(4):799-803. Epub 2007 Dec 6. Review.
Lebbink RJ, de Ruiter T, Kaptijn GJ, Bihan DG, Jansen CA, Lenting PJ, Meyaard L.
Mouse leukocyte-associated Ig-like receptor-1 (mLAIR-1) functions as an inhibitory collagen-binding receptor on immune cells. Int Immunol. 2007 Aug;19(8):1011-9. Epub 2007 Aug 16. (113, FC, WB, IP, Pubmed)
Meyaard L, Adema GJ, Chang C, Woollatt E, Sutherland GR, Lanier LL, Phillips JH.LAIR-1, a novel inhibitory receptor expressed on human mononuclear leukocytes.
Immunity. 1997 Aug;7(2):283-90.
