Contents: Phycoerythrin (PE) anti-mouse MIG
Catalog Number: 12-3009
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MIG-2F5.5
Isotype: Armenian Hamster IgG
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Staining of LPS-stimulated (open histogram) and IFN-g-stimulated (colored histogram) RAW 246.7 cells with PE anti-mouse MIG (MIG-2F5.5). Total viable cells were used for analysis.
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Description
The monoclonal antibody MIG-2F5.5 reacts with mouse monokine induced by IFN-g (mig, mig-1, CXCL9). This 14.4 kDa inflammatory chemokine is specifically induced by IFN-g, but not other activators such as LPS or IFN-a. Secretion of mig, mainly by macrophages results in the chemotaxis of a variety of activated T cells via the CXCR3 chemokine receptor. Mig is involved many areas of research including autoimmune diseases, cancer, and inflammation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This MIG-2F5.5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested
This MIG-2F5.5 antibody has been tested by intracellular staining followed by flow cytometric analysis of IFN-g stimulated thioglycolate PEC and RAW 264.7 cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Lu H, et al. 2007. Expression and purification of bioactive high-purity mouse monokine induced by IFN-gamma in Escherichia coli.
Protein Expr Purif. 2007 Sep;55(1):132-8. Epub 2007 Apr 14.
Wong P, et al. 1994.
A unique palindromic element mediates gamma interferon induction of mig gene expression.
Mol. Cell. Biol. 14: 914-922
F Liao, et al. 1995.
Human Mig chemokine: biochemical and functional characterization.
J. Exp. Med. 182: 1301-1314.
Klunker S, et al. 2003.
A Second Step of Chemotaxis After Transendothelial Migration: Keratinocytes Undergoing Apoptosis Release IFN-{gamma}-Inducible Protein 10, Monokine Induced by IFN-{gamma}, and IFN-{gamma}-Inducible {alpha}-Chemoattractant for T Cell Chemotaxis Toward Epidermis in Atopic Dermatitis.
J Immunol 171: 1078-1084
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