Product Information

Contents: Phycoerythrin (PE) Human PD-1 (CD279)
Catalog Number: 12-2799
Sizes: 25 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioJ105 (J105)
Isotype: Mouse IgG1, κ

Staining of 3-day PHA activated PBMCs with PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or PE Human PD-1 (J105) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-2799	Affinity Purified anti-human PD-1 (CD279, PD1)	N/A	N/A	FC IH/F IP
13-2799	Biotin anti-human PD-1 (CD279, PD1)	N/A	N/A	FC
12-2799	Phycoerythrin (PE) anti-human PD-1 (CD279, PD1)	488	575	FC
17-2799	Allophycocyanin (APC) anti-human PD-1 (CD279, PD1)	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells.
Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (cat. 11-9969).

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

This eBioJ105 (J105) antibody has been pre-titrated and tested by flow cytometic analysis of PHA stimulated PBMC. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Iwai Y, Okazaki T, Nishimura H, Kawasaki A, Yagita H, Honjo T. Microanatomical localization of PD-1 in human tonsils. Immunol Lett. 2002 Oct 1;83(3):215-20. PubMed

Related Products

Cat. 12-4714  Phycoerythrin (PE) Mouse IgG1, K Isotype Control
Cat. 12-9969  Phycoerythrin (PE) anti-human PD-1 (CD279, PD1) (clone MIH4)
Cat. 12-9981  Phycoerythrin (PE) anti-mouse PD-1 (CD279, PD1) (clone RMP1-30)
Cat. 12-9985  PE anti-mouse PD-1 (CD279, PD1) (clone J43)