Product Information

Contents: Phycoerythrin (PE) anti-human CD182 (CXCR2, IL-8RB)
Catalog Number: 12-1829
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio5E8-C7-F10 (5E8-C7-F10)
Isotype: Mouse IgG1, κ

Staining of normal human peripheral blood cells with APC anti-human CD4 (OKT4) (cat. 17-0048) (left) or APC anti-human CD56 (cat. 17-0569) (right) and PE anti-human CD182 (5E8-C7-F10). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1829	Affinity Purified anti-human CD182 (CXCR2, IL-8RB, IL8RB)	N/A	N/A	FC IHC
12-1829	Phycoerythrin (PE) anti-human CD182 (CXCR2, IL-8RB, IL8RB)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8Rβ). CD182 is a 67-70 kDa member of the seven-transmembrane spanning G-protein coupled receptor (GPCR) family. CD182 is expressed as a homodimer, or a heterodimer with CD181 (CXCR1, IL-8Rα) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO-α, -β and -γ, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Jones SA, Dewald B, Clark-Lewis I, Baggiolini M. Chemokine antagonists that discriminate between interleukin-8 receptors. Selective blockers of CXCR2. J Biol Chem. 1997 Jun 27;272(26):16166-9.

Petering H, Gotze O, Kimmig D, Smolarski R, Kapp A, Elsner J. The biologic role of interleukin-8: functional analysis and expression of CXCR1 and CXCR2 on human eosinophils. Blood. 1999 Jan 15;93(2):694-702.

Addison CL, Daniel TO, Burdick MD, Liu H, Ehlert JE, Xue YY, Buechi L, Walz A, Richmond A, Strieter RM. The CXC chemokine receptor 2, CXCR2, is the putative receptor for ELR+ CXC chemokine-induced angiogenic activity. J Immunol. 2000 Nov 1;165(9):5269-77.

Chuntharapai A, Lee J, Hebert CA, Kim KJ. Monoclonal antibodies detect different distribution patterns of IL-8 receptor A and IL-8 receptor B on human peripheral blood leukocytes. J Immunol. 1994 Dec 15;153(12):5682-8.

Sprenger H, Lloyd AR, Lautens LL, Bonner TI, Kelvin DJ. Structure, genomic organization, and expression of the human interleukin-8 receptor B gene. J Biol Chem. 1994 Apr 15;269(15):11065-72.

Related Products

Cat. 11-1819 Fluorescein isothiocyanate (FITC) anti-human CD181 (CXCR1, IL-8RA, IL8RA) (clone eBio8F1-1-4 (8F1-1-4))
Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control