Product Information

Contents: Phycoerythrin (PE) anti-human CD180 (RP105, Toll-like Receptor/ TLR Family)
Catalog Number: 12-1809
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MHR73-11
Isotype: Mouse IgG1, κ
HLDA No.: N/A

Surface staining of normal human peripheral blood cells with anti-human CD180 (MHR73-11) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1809	Affinity Purified anti-human CD180 (RP105, Toll-like Receptor/ TLR Family)	N/A	N/A	FA FC IHC IP
16-1809	Functional Grade* Purified anti-human CD180 (RP105, Toll-like Receptor/ TLR Family)	N/A	N/A	FA FC
13-1809	Biotin anti-human CD180 (RP105, Toll-like Receptor/ TLR Family)	N/A	N/A	FC
12-1809	PE anti-human CD180 (RP105, Toll-like Receptor/ TLR Family)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MHR73-11 monoclonal antibody reacts with human CD180 (RP105). This 105 kDa type I transmembrane molecule is a member of the TLR family of proteins characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. RP105 physically associates with another molecule called MD-1 and is expressed on B, monocytes/macrophages, and dendritic cells. Histological studies show that RP105 is expressed mainly on mature B cells in mantle zones, while germinal center cells are either dull or negative. The RP105/MD-1 complex in concert with TLR4 mediates B cell recognition and signaling of LPS. MHR73-11 activates B cells, leading to increases in cell size, expression of the costimulatory molecule CD80, and DNA synthesis. Moreover, ligation of RP105 protects B cells from irradiation- or dexamethasone-induced apoptosis. Thus, RP105 is a signal transduction molecule and plays a role in regulation of B cell growth and death. A significant proportion of circulating B cells in SLE patients is RP105 negative. Loss of RP105 is associated with B cell activation and increased disease activity in SLE patients.

Applications Reported

For research use only, not for diagnostic or therapeutic use. MHR73-11 has been reported for use in flow cytometric analysis.

Applications Tested

The MHR73-11 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Koarada, S., Y. Tada, et al. 1999. B cells lacking RP105, a novel B cell antigen, in systemic lupus erythematosus. Arthritis Rheum 42(12): 2593-600.
Miura, Y., K. Miyake, et al. 1996. Molecular cloning of a human RP105 homologue and chromosomal localization of the mouse and human RP105 genes (Ly64 and LY64). Genomics 38(3): 299-304.
Miura, Y., R. Shimazu, et al. 1998. RP105 is associated with MD-1 and transmits an activation signal in human B cells. Blood 92(8): 2815-22.

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