Product Information

Contents: Phycoerythrin (PE) anti-human CD154
Catalog Number: 12-1548
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE
LIGHT-SENSITIVE MATERIAL
Clone: 24-31
Isotype: Mouse IgG1, κ
HLDA No.: N/A

Staining of 6 hour PMA/ionomycin stimulated PBMC's with PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or PE anti-human CD154 (24-31) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1548	Affinity Purified anti-human CD154 (CD40 Ligand, CD40L)	N/A	N/A	FA FC
16-1548	Functional Grade* Purified anti-human CD154 (CD40 Ligand, CD40L)	N/A	N/A	FA FC
11-1548	FITC anti-human CD154 (CD40 Ligand, CD40L)	488	518	FC
12-1548	PE anti-human CD154 (CD40 Ligand, CD40L)	488	575	FC
17-1548	Allophycocyanin (APC) anti-human CD154 (CD40 Ligand, CD40L)	633	660	FC
47-1548	Allophycocyanin-eFluor^TM 780 anti-human CD154 (CD40 Ligand, CD40L)	633	780	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the Cynomogus monkey CD154.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 24-31 has been reported for use in flow cytometric analysis.

Applications Tested

The 24-31 antibody has been pretitrated and tested by flow cytometric analysis of resting and activated human PBMC and can be used at 20 µl (0.5 μg) per test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Brams, P., A. Black, et al. 2001. A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation. Int Immunopharmacol 1(2): 277-94.

Related Products

Cat. 11-4718 FITC Mouse IgG1, K Isotype Control (I/C Staining)