Product Information

Contents: Phycoerythrin (PE) anti-human CD152 (CTLA-4, CTLA4)
Catalog Number: 12-1528
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio20A (20A, A3)
Isotype: Mouse IgG2a, κ

Intracellular staining of 3-day PHA-stimulated normal human peripheral blood mononuclear cells with APC anti-human CD3 (UCHT1) (cat. 17-0038) and PE Mouse IgG2a, K Iso Cntrl (cat. 12-4724) (left) or PE anti-human CD152 (20A) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-1528	Phycoerythrin (PE) anti-human CD152 (CTLA-4, CTLA4)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio20A monoclonal antibody reacts with human CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells (activated B cells may also express CTLA-4) and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in intracellular localization of a large proportion of newly synthesized CTLA-4 in response to TCR signaling.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio20A (20A, A3) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio20A (20A, A3) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of PHA stimulated human PBMCs. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Vandenborre K, et al. American Journal of Pathology (1998) 152: 963-73.
Vandenborre K, et al. Immunology (1999) 98: 1-10.
T.L. Walunas, et al. Immunity (1994) 1: 405-413.
N.J. Karandikar, et al. Journal of Experimental Medicine (1996) 184: 783-788.

Related Products

Cat. 12-1520  Phycoerythrin (PE) anti-rat CD152 (CTLA-4, CTLA4) (clone WKH203)
Cat. 12-1522  PE anti-mouse CD152 (CTLA-4, CTLA4) (clone UC10-4B9)
Cat. 12-1529  PE anti-human CD152 (CTLA-4, CTLA4) (clone 14D3)
Cat. 12-4724  Phycoerythrin (PE) Mouse IgG2a, K Isotype Control