Product Information

Contents: Phycoerythrin (PE) anti-human CD150 (SLAM, IPO-3)
Catalog Number: 12-1509
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: A12 (7D4)
Isotype: Mouse IgG1, κ
HLDA No.: N/A

Staining of normal human peripheral blood cells with PE mouse IgG1 isotype control (cat. 12-4719) (open histogram) or PE A12 (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1509	Affinity Purified anti-human CD150 (SLAM, IPO-3, IPO3)	N/A	N/A	FA FC IHC
16-1509	Functional Grade* Purified anti-human CD150 (SLAM, IPO-3, IPO3)	N/A	N/A	FA FC
13-1509	Biotin anti-human CD150 (SLAM, IPO-3, IPO3)	N/A	N/A	FC
11-1509	FITC anti-human CD150 (SLAM, IPO-3, IPO3)	488	518	FC
12-1509	PE anti-human CD150 (SLAM, IPO-3, IPO3)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The A12 monoclonal antibody reacts with human CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM induces proliferation and cytokine production of T cells and SLAM plays a role in activation of T and B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. A12 (7D4) has been reported for use in flow cytometric analysis.

Applications Tested

The A12 (7D4) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Cocks, B. G., C. C. Chang, et al. (1995). "A novel receptor involved in T-cell activation." Nature 376(6537): 260-3.

Related Products

Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control