Product Information

Contents: Phycoerythrin (PE) anti-mouse Ly108 receptor (SLAM Family receptor)
Catalog Number: 12-1508
Sizes: 25 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio13G3-19D (13G3-19D)
Isotype: Mouse IgG2a, κ

Staining of BALB/c splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.06 μg of PE Mouse IgG2a, K Iso Cntrl (cat. 12-4724) (left) or 0.06 μg of PE anti-mouse Ly108 receptor (SLAM Family receptor) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-1508	Phycoerythrin (PE) anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108)	488	575	FC
13-1508	Biotin anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108)	N/A	N/A	FC
14-1508	Affinity Purified anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108)	N/A	N/A	FC IP WB
16-1508	Functional Grade* Purified anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108)	N/A	N/A	FA FC IP WB
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is also known as NTB-A (NK cell, T cell, B cell antigen), and is expressed on NK, T cells, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and expression of IFN-gamma, and contributes to the inability of NK cells to kill Epstein Barr virus-infected B cells in X-linked lymphoproliferative disease. CD4+ T cells from Ly108 knock-out mice exhibit diminished ability to produce IL-4 and defective neutrophil functions.

Staining has not revealed any strain differences between C57BL/6 and BALB/c mice. Other strains have not been tested.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio13G3-19D (13G3-19D) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio13G3-19D (13G3-19D) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Peck SR, Ruley HE. Ly108: a new member of the mouse CD2 family of cell surface proteins. Immunogenetics. 2000 Nov;52(1-2):63-72.

Fraser CC, Howie D, Morra M, Qiu Y, Murphy C, Shen Q, Gutierrez-Ramos JC, Coyle A, Kingsbury GA, Terhorst C. Identification and characterization of SF2000 and SF2001, two new members of the immune receptor SLAM/CD2 family. Immunogenetics. 2002 Feb;53(10-11):843-50.

Howie D, Laroux FS, Morra M, Satoskar AR, Rosas LE, Faubion WA, Julien A, Rietdijk S, Coyle AJ, Fraser C, Terhorst C. Cutting edge: the SLAM family receptor Ly108 controls T cell and neutrophil functions. J Immunol. 2005 May 15;174(10):5931-5.

Related Products

Cat. 12-4724 Phycoerythrin (PE) Mouse IgG2a, K Isotype Control