Contents: Phycoerythrin (PE) anti-mouse Ly108 receptor (SLAM Family receptor)
Catalog Number: 12-1508
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio13G3-19D (13G3-19D)
Isotype: Mouse IgG2a, κ
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Price |
Qty |
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*International customers: Please contact your distributor for region specific pricing.
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Staining of BALB/c splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.06 μg of PE Mouse IgG2a, K Iso Cntrl (cat. 12-4724) (left) or 0.06 μg of PE anti-mouse Ly108 receptor (SLAM Family receptor) (right). Cells in the lymphocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 12-1508 |
Phycoerythrin (PE) anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108) |
488 |
575 |
FC
|
| 13-1508 |
Biotin anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108) |
N/A |
N/A |
FC
|
| 14-1508 |
Affinity Purified anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108) |
N/A |
N/A |
FC
IP
WB
|
| 16-1508 |
Functional Grade* Purified anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108) |
N/A |
N/A |
FA
FC
IP
WB
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is also known as NTB-A (NK cell, T cell, B cell antigen), and is expressed on NK, T cells, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and expression of IFN-gamma, and contributes to the inability of NK cells to kill Epstein Barr virus-infected B cells in X-linked lymphoproliferative disease. CD4+ T cells from Ly108 knock-out mice exhibit diminished ability to produce IL-4 and defective neutrophil functions.
Staining has not revealed any strain differences between C57BL/6 and BALB/c mice. Other strains have not been tested.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio13G3-19D (13G3-19D) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio13G3-19D (13G3-19D) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Peck SR, Ruley HE.
Ly108: a new member of the mouse CD2 family of cell surface proteins.
Immunogenetics. 2000 Nov;52(1-2):63-72.
Fraser CC, Howie D, Morra M, Qiu Y, Murphy C, Shen Q, Gutierrez-Ramos JC, Coyle A, Kingsbury GA, Terhorst C.
Identification and characterization of SF2000 and SF2001, two new members of the immune receptor SLAM/CD2 family.
Immunogenetics. 2002 Feb;53(10-11):843-50.
Howie D, Laroux FS, Morra M, Satoskar AR, Rosas LE, Faubion WA, Julien A, Rietdijk S, Coyle AJ, Fraser C, Terhorst C.
Cutting edge: the SLAM family receptor Ly108 controls T cell and neutrophil functions.
J Immunol. 2005 May 15;174(10):5931-5.
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