Contents: Phycoerythrin (PE) anti-mouse CD150 (SLAM, IPO-3)
Catalog Number: 12-1501
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 9D1
Isotype: Rat IgG1
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.25 μg of PE Rat IgG1 Iso Cntrl (cat. 12-4301) (left) or 0.25 μg of PE anti-mouse CD150 (9D1) (right). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-1501 |
Affinity Purified anti-mouse CD150 (SLAM, IPO-3) |
N/A |
N/A |
FA
FC
|
| 16-1501 |
Functional Grade* Purified anti-mouse CD150 (SLAM, IPO-3) |
N/A |
N/A |
FA
FC
|
| 13-1501 |
Biotin anti-mouse CD150 (SLAM, IPO-3) |
N/A |
N/A |
FC
|
| 11-1501 |
Fluorescein isothiocyanate (FITC) anti-mouse CD150 (SLAM, IPO-3) |
488 |
518 |
FC
|
| 12-1501 |
Phycoerythrin (PE) anti-mouse CD150 (SLAM, IPO-3) |
488 |
575 |
FC
|
| 17-1501 |
Allophycocyanin (APC) anti-mouse CD150 (SLAM, IPO-3) |
633 |
660 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 9D1 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T (especially TH1) and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. 9D1 is reported to be an activating antibody.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 9D1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 9D1 antibody has been tested by flow cytometric analysis of mouse thymic and splenic cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Howie D, Okamoto S, Rietdijk S, Clarke K, Wang N, Gullo C, Bruggeman JP, Manning S, Coyle AJ, Greenfield E, Kuchroo V, Terhorst C. 2002. The role of SAP in murine CD150 (SLAM)-mediated T-cell proliferation and interferon gamma production. Blood. 100(8): 2899-907. (9D1, FC, FA,
PubMed)
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Cat.
12-4301 PE Rat IgG1 Isotype Control
Mouse Cellular Antigens Chart