Contents: Phycoerythrin (PE) anti-human CD144 (VE-Cadherin)
Catalog Number: 12-1449
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 16B1
Isotype: Mouse IgG1
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Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.5 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or 0.5 μg of PE anti-human CD144 (VE-Cadherin) (colored histogram). Total viable cells were used for analysis.
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Description
The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 16B1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 16B1 antibody has been tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF.
Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
Stem Cells. 2007 Feb;25(2):490-9. (
16B1, Immunocytofluorescence,
PubMed)
Suzuki S, Sano K, and Tanihara H. 1991. Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue.
Cell Regul 2: 261-270.
Breviario F, Caveda L, Corada M, Martin-Padura I, Navarro P, Golay J, Introna M, Gulino D, Lampugnani MG, and Dejana E. 1995. Functional properties of human vascular endothelial cadherin (7B4/Cadherin-5), an endothelium-specific cadherin.
Arterioscler Thromb Vasc Biol 15: 1229-1239.
Vincent PA, Xiao K, Buckley KM, and Kowalczyk AP. 2004. VE-Cadherin: adhesion at arm's length.
Am J Physiol Cell Physiol 286: 987-997.
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12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart