Staining of the Jurkat (left) and A375 (right) cell lines with Mouse IgG1 κ Isotype Control PE (cat. 12-4714) (blue histogram) or Anti-Human Oncostatin M Receptor PE (purple histogram). Total viable cells were used for analysis.
Contents: Anti-Human Oncostatin M Receptor PE Catalog Number: 12-1303 Concentration: 5 μl (0.5 μg)/test Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: AN-V2 Host/Isotype: Mouse IgG1
Product Options
Cat. No.
Size
Price
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12-1303-41
25 tests
12-1303-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
This monoclonal AN-V2 antibody reacts with the human oncostatin M (OSM) receptor. OSM, which belongs to the interleukin-6 cytokine family, is produced by T cells, activated monocytes, and dendritic cells. In humans, OSM can bind two unique receptor complexes. The type I OSM receptor is composed of the LIFR associated with gp130, and recognizes both LIF and OSM. The type II OSM receptor, which consists of gp130 and the OSMR subunit, specifically binds the cytokine, leading to activation of the JAK/STAT signaling pathway. Reports have also indicated a soluble form of the OSM receptor, which this antibody also recognizes. The OSM receptor is expressed in the bone marrow, endothelium, liver, lung, and numerous tumor cell lines.
The AN-V2 monoclonal antibody recognizes the type II (beta) OSM receptor.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This AN-V2 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This AN-V2 antibody has been pre-titrated and tested by flow cytometric analysis of the A375 cell line. This can be used at 5 μl (0.5 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Diveu C, Venereau E, Froger J, Ravon E, Grimaud L, Rousseau F, Chevalier S, Gascan H. Molecular and functional characterization of a soluble form of oncostatin M/interleukin-31 shared receptor. J Biol Chem. 2006 Dec 1;281(48):36673-82.
(AN-V2, FC, Pubmed)
Gómez-Lechón MJ. Oncostatin M: signal transduction and biological activity. Life Sci. 1999;65(20):2019-30.
