Product Information
Contents: Phycoerythrin (PE) anti-mouse CD127 (IL-7 Receptor α, IL-7Rα)
Catalog Number: 12-1271
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: A7R34
Isotype: Rat IgG2a, κ
|
|
2-color staining of mouse (C57BL/6) splenocytes with FITC anti-mouse CD3e (145-2C11)(cat.no. 11-0031) and 0.125 μg PE anti-mouse CD127 (A7R34)
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-1271 |
FITC anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
488 |
518 |
FC
|
| 12-1271 |
PE anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
488 |
575 |
FC
|
| 13-1271 |
Biotin anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
N/A |
N/A |
FC
|
| 14-1271 |
Affinity Purified anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
N/A |
N/A |
FA
FC
IH/F
IP
|
| 15-1271 |
PE-Cy5 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
488 |
670 |
FC
|
| 16-1271 |
Functional Grade* Purified anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
N/A |
N/A |
FA
FC
|
| 17-1271 |
APC anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
633 |
660 |
FC
|
| 25-1271 |
PE-Cy7 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
488 |
760 |
FC
|
| 27-1271 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 45-1271 |
PerCP-Cy5.5 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
488 |
690 |
FC
|
| 51-1271 |
Alexa Fluor® 647 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
633 |
668 |
FC
|
| 53-1271 |
Alexa Fluor® 488 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
488 |
519 |
FC
|
| 56-1271 |
Alexa Fluor® 700 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
| 57-1271 |
Pacific Blue® anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) |
402 |
421 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity α subunit of the mouse IL-7 receptor. IL-7 receptor α chain is expressed by immature B cells in the bone marrow, double-negative (CD4
-/CD8
-), single-positive (CD4
+ and CD8
+), but not double-positive (CD4
+/CD8
+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in
in vivo and
in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The A7R34 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The A7R34 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Sudo, T., S. Nishikawa, et al. 1993. Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc Natl Acad Sci U S A 90(19): 9125-9.
Ohana M, Okazaki K, Oshima C, et al. 2001. A critical role for IL-7R signaling in the development of Helicobacter felis-induced gastritis in mice. Gastroenterology. Aug;121(2):329-36.
Okuno Y, Iwasaki H, Huettner CS, Radomska HS, Gonzalez DA, Tenen DG, Akashi K. 2002. Differential regulation of the human and murine CD34 genes in hematopoietic stem cells. Proc Natl Acad Sci U S A. Apr 30;99(9):6246-51.
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