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Mouse Cellular Antigens Chart

Contents: Phycoerythrin (PE) anti-mouse CD122 (Interleukin-2 Receptor β, IL-2Rβ) Catalog Number: 12-1222 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. Clone: TM-b1 (TM-beta1) Isotype: Rat IgG2b Prices for This Product* Cat. No. Size Price Qty Action 12-1222-81 50 ug $120 12-1222-82 100 ug $220 12-1222-83 200 ug $350 *International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with APC anti-mouse NK-1.1 (PK136) (cat. 17-5941) and .06 μg of PE Rat IgG2b Isotype Control (cat. 12-4031) (left) or .06 μg of PE anti-mouse CD122 (TM-b1) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1222	Purified anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	N/A	N/A	FC  IP  NU
16-1222	Functional Grade* Purified anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	N/A	N/A	FA  FC
13-1222	Biotin anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	N/A	N/A	FC
11-1222	Fluorescein isothiocyanate (FITC) anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	488	518	FC
12-1222	Phycoerythrin (PE) anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	488	575	FC
57-1222	Pacific Blue® anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The TM-β1 monoclonal antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor β chain (IL-2Rβ). CD122 is expressed by NK cells and some T cells in the periphery, and is upregulated by activation. CD122 associates with CD25 and CD132 (common γ chain) to form the high affinity IL-2 receptor. High affinity binding of IL-2 to the IL-2 receptor is inhibited by the TM-β1 antibody.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The TM-β1 antibody has been reported for use in flow cytometric analysis.

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Applications Tested

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This TM-b1 (TM-beta1) antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Takeuchi Y, Tanaka T, Hamamura K, Sugimoto T, Miyasaka M, Yagita H, Okumura K. 1992. Expression and role of interleukin-2 receptor beta chain on CD4-CD8- T cell receptor alpha beta+ cells. Eur J Immunol. 22(11):2929-35.

Tanaka T, Takeuchi Y, Shiohara T, Kitamura F, Nagasaka Y, Hamamura K, Yagita H, Miyasaka M. 1992. In utero treatment with monoclonal antibody to IL-2 receptor beta-chain completely abrogates development of Thy-1+ dendritic epidermal cells. Int Immunol. 4(4):487-91.

French JD, Roark CL, Born WK, O'brien RL. {gamma}{delta} T cell homeostasis is established in competition with {alpha}{beta} T cells and NK cells. Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14741-6. Epub 2005 Oct 3. (blocking, PubMed)

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Related Products

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Cat. 12-1221    PE anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb) (clone 5H4)
Cat. 13-1221    Biotin anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb) (clone 5H4)
Cat. 14-1221    Affinity Purified anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb) (clone 5H4)
Cat. 16-1221    Functional Grade Purified anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb) (clone 5H4)