Product Information

Contents: Phycoerythrin (PE) anti-human CD119 (IFNgR1 alpha chain)
Catalog Number: 12-1199
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: GIR-208 (GIR 208)
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with 0.5 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (open histogram) or PE anti-human CD119 (GIR-208) (colored histogram). Cells in the lymphocyte gate (left) or monocytes (right) were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-1199	Phycoerythrin (PE) anti-human CD119 (IFNgR1 alpha chain)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody reacts with human CD119 also known as the IFN-γ receptor 1 (alpha chain). IFN γR1 is found in a complex with IFN-γ receptor beta and recognizes with high affinity IFNγ. Expression is ubiquitous but low, although some cell types, such as monocytes express higher levels.
The GIR-208 monoclonal antibody is blocked from binding when IFNγ is bound to the receptor.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This GIR-208 (GIR 208) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This GIR-208 (GIR 208) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

Sheehan KC, Calderon J, Schreiber RD. Generation and characterization of monoclonal antibodies specific for the human IFN-gamma receptor. J Immunol. 1988 Jun 15;140(12):4231-7. (GIR-208, FC, FA PubMed)

Related Products

Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control