Contents: Phycoerythrin (PE) anti-mouse CD117 (c-Kit, cKit)
Catalog Number: 12-1171
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 2B8
Isotype: Rat IgG2b, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of C57Bl/6 bone marrow cells with FITC anti-mouse/human CD45R (B220)(RA3-6B2)(cat. 11-0452) and 0.06 μg PE Rat IgG2b Iso Cntrl (cat. 12-4031) (left) or 0.06 μg PE anti-mouse CD117 (2B8) (right). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-1171 |
Affinity Purified anti-mouse CD117 (c-Kit, cKit) |
N/A |
N/A |
FC
IP
|
| 13-1171 |
Biotin anti-mouse CD117 (c-Kit, cKit) |
N/A |
N/A |
FC
|
| 11-1171 |
FITC anti-mouse CD117 (c-Kit, cKit) |
488 |
518 |
FC
|
| 12-1171 |
PE anti-mouse CD117 (c-Kit, cKit) |
488 |
575 |
FC
|
| 15-1171 |
PE-Cy5 anti-mouse CD117 (c-Kit, cKit) |
488 |
670 |
FC
|
| 17-1171 |
APC anti-mouse CD117 (c-Kit, cKit) |
633 |
660 |
FC
|
| 19-1171 |
Cy5 anti-mouse CD117 (c-Kit, cKit) |
633 |
670 |
FC
|
| 25-1171 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD117 (c-Kit, cKit) |
488 |
760 |
FC
|
| 35-1171 |
Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD117 (c-Kit, cKit) |
488 |
690 |
FC
|
| 27-1171 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD117 (c-Kit, cKit) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1
lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 2B8 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ikuta K, Weissman IL. 1992. Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation. Proc Natl Acad Sci USA. 89(4): 1502-6.
Related Products
Cat.
12-4031 Phycoerythrin (PE) Rat IgG2b Isotype Control
Mouse Cellular Antigens Chart