Product Information

Contents: Phycoerythrin (PE) anti-human CD115 (CSF-1R, FMS, CSF-1 Receptor)
Catalog Number: 12-1159
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 12-3A3-1B10
Isotype: Rat IgG1

Staining of normal human peripheral blood cells with PE Rat IgG1 Iso Cntrl (cat. 12-4301) (open histogram) or PE anti-human CD115 (12-3A3-1B10) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1159	Affinity Purified anti-human CD115 (CSF-1R, FMS, CSF-1 Receptor)	N/A	N/A	FC IHC IP WB
13-1159	Biotin anti-human CD115 (CSF-1R, FMS, CSF-1 Receptor)	N/A	N/A	FC
12-1159	Phycoerythrin (PE) anti-human CD115 (CSF-1R, FMS, CSF-1 Receptor)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 12-3A3-1B10 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 12-3A3-1B10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 20 μl (0.03 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Ashmun RA, Look AT, Roberts WM, Roussel MF, Seremetis S, Ohtsuka M, Sherr CJ. Monoclonal antibodies to the human CSF-1 receptor (c-fms proto-oncogene product) detect epitopes on normal mononuclear phagocytes and on human myeloid leukemic blast cells. Blood. 1989 Feb 15;73(3):827-37. (12-3A3-1B10, FC, PubMed)

Pixley FJ, Stanley ER. CSF-1 regulation of the wandering macrophage: complexity in action. Trends Cell Biol. 2004 Nov;14(11):628-38. Review.

Related Products

Cat. 12-4301 PE Rat IgG1 Isotype Control