Contents: Phycoerythrin (PE) anti-mouse CD115
Catalog Number: 12-1152
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: AFS98
Isotype: Rat IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of C57Bl/6 peritoneal exudate cells with 0.03 μg PE Rat IgG2a Iso Cntrl (cat. 12-4321) (open histogram) or 0.03 μg PE anti-mouse CD115 (AFS98) (colored histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-1152 |
Affinity Purified anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor) |
N/A |
N/A |
FA
FC
IH/F
WB
|
| 16-1152 |
Functional Grade* Purified anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor) |
N/A |
N/A |
FA
FC
|
| 13-1152 |
Biotin anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor) |
N/A |
N/A |
FC
|
| 12-1152 |
PE anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor) |
488 |
575 |
FC
|
| 17-1152 |
Allophycocyanin (APC) anti-mouse CD115 (FMS, CSF-1R, CSF-1 receptor) |
633 |
660 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The AFS98 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The AFS98 antibody has been tested by flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Murayama T, Yokode M, et al. 1999. Intraperitoneal administration of anti-c-fms monoclonal antibody prevents initial events of atherogenesis but does not reduce the size of advanced lesions in apolipoprotein E-deficient mice. Circulation. 99(13): 1740-6.
Sudo T, Nishikawa S, et al. 1995. Functional hierarchy of c-kit and c-fms in intramarrow production of CFU-M. Oncogene. 11(12): 2469-76.
Related Products
Cat.
12-4321 PE Rat IgG2a Isotype Control
Mouse Cellular Antigens Chart