Product Information

Contents: Phycoerythrin (PE) anti-rat CD103 (Integrin alphaE2, aE2, DC marker)
Catalog Number: 12-1030
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OX62
Isotype: Mouse IgG1

Staining of Lou rat splenocytes with FITC anti-rat CD4 (OX35) (cat. 11-0040) and 0.125 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or 0.125 μg of PE anti-rat CD103 (OX62) (right). Cells in the lymphocyte gate were used for analysis and events are displayed after gating out CD3+ T cells and CD45R+ B cells, and gating on the remaining cells which are MHC Class II+.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-1030	Phycoerythrin (PE) anti-rat CD103 (Integrin alphaE2, aE2, DC marker)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The OX62 monoclonal antibody reacts with rat CD103, also known as αE2 integrin. CD103 was originally identified on rat veiled dendritic cells, and is expressed by dendritic epidermal T cells in the skin, intraepithelial lymphocytes in the small intestine and by dendritic cells associated with γδ T cells. Integrins are a family of heterodimeric proteins which contain non-covalently associated α and β transmembrane glycoproteins. Integrins function as receptors for extracellular matrix and cell-cell adhesion molecules.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OX62 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This OX62 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Brenan M, Puklavec M. The MRC OX-62 antigen: a useful marker in the purification of rat veiled cells with the biochemical properties of an integrin. J Exp Med. 1992 Jun 1;175(6):1457-65. (OX62, mAb development, FC, PubMed)

Voisine C, Hubert FX, Trinité B, Heslan M, Josien R. Two phenotypically distinct subsets of spleen dendritic cells in rats exhibit different cytokine production and T cell stimulatory activity. J Immunol. 2002 Sep 1;169(5):2284-91. (OX62, FC, PubMed)

Hubert FX, Voisine C, Louvet C, Heslan M, Josien R. Rat plasmacytoid dendritic cells are an abundant subset of MHC class II+ CD4+CD11b-OX62- and type I IFN-producing cells that exhibit selective expression of Toll-like receptors 7 and 9 and strong responsiveness to CpG. J Immunol. 2004 Jun 15;172(12):7485-94. (OX62, FC, PubMed)

Related Products

Cat. 12-1031  PE anti-mouse CD103 (Integrin alpha-IEL chain) (clone 2E7)
Cat. 12-1038  PE anti-human CD103 (Integrin alpha-IEL chain) (clone B-Ly7)
Cat. 12-4714  Phycoerythrin (PE) Mouse IgG1, K Isotype Control