Product Information

Contents: Phycoerythrin (PE) anti-human ICAM-2 (CD102)
Catalog Number: 12-1029
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CBRIC2/2
Isotype: Mouse IgG2a

Staining of normal human peripheral blood cells with PE Mouse IgG2a, K Iso Cntrl (cat. 12-4724) (open histogram) or PE anti-human ICAM-2 (CBRIC2/2) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1029	Affinity Purified anti-human ICAM-2 (CD102)	N/A	N/A	FA FC IHC IP WB
11-1029	Fluorescein isothiocyanate (FITC) anti-human ICAM-2 (CD102)	488	518	FC
12-1029	Phycoerythrin (PE) anti-human ICAM-2 (CD102)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The CBRIC2/2 monoclonal antibody reacts with human ICAM-2 (CD102). ICAM-2 is a 55-65 kD transmembrane glycoprotein possessing 2 extracellular Ig domains, a single transmembrane domain, and a short 26-amino acid cytoplasmic domain. ICAM-2 is expressed on most leukocytes, and is strongly expressed on vascular endothelial cells. ICAM-2 is a ligand of integrin CD11a/CD18 (LFA-1), and also has been reported to bind integrin CD11b/CD18 (Mac-1). ICAM-2 is an intercellular adhesion molecule involved in lymphocyte homing to sites of inflammation. The CBRIC2/2 antibody has been reported to have blocking activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This CBRIC2/2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This CBRIC2/2 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at 20 μl (0.06 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

de Fougerolles AR, Stacker SA, Schwarting R, Springer TA. Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1. J Exp Med. 1991 Jul 1;174(1):253-67. (CBRIC2/2, FC, IP, FA, PubMed)

Diacovo TG, deFougerolles AR, Bainton DF, Springer TA. A functional integrin ligand on the surface of platelets: intercellular adhesion molecule-2. J Clin Invest. 1994 Sep;94(3):1243-51. (CBRIC2/2, FC, IP, FA, PubMed)

Butini L, De Fougerolles AR, Vaccarezza M, Graziosi C, Cohen DI, Montroni M, Springer TA, Pantaleo G, Fauci AS. Intercellular adhesion molecules (ICAM)-1 ICAM-2 and ICAM-3 function as counter-receptors for lymphocyte function-associated molecule 1 in human immunodeficiency virus-mediated syncytia formation. Eur J Immunol. 1994 Sep;24(9):2191-5. (CBRIC2/2, FA, PubMed)

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Cat. 12-4724  Phycoerythrin (PE) Mouse IgG2a, K Isotype Control