Product Information

Contents: Phycoerythrin (PE) anti-rat IgM
Catalog Number: 12-0990
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIS40
Isotype: Mouse IgG1, κ

Surface staining of rat splenocytes with anti-rat IgM (HIS40) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0990	Affinity Purified anti-rat IgM	N/A	N/A	FC IHC
11-0990	FITC anti-rat IgM	488	518	FC
12-0990	PE anti-rat IgM	488	575	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HIS40 monoclonal antibody reacts with the μ heavy chain of rat IgM. It does not react with other classes of rat immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated HIS40 is used as a detection secondary for rat IgM primary antibodies.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIS40 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HIS40 antibody has been tested by flow cytometric analysis as a second step to detect rat IgM primary antibodies. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kroese, FGM. 2001. Personal communication. Kroese FG, Butcher EC, et al. 1990. The rat B cell system: the anatomical localization of flow cytometry-defined B cell subpopulations. Eur J Immunol. 20(7): 1527-34. Kroese, FG, Wubbena AS, et al. 1987. B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens. Eur J Immunol. 17(7): 921-8.

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